Abstract: Based on the published nucleotide sequence of porcine epidemic diarrhea virus N gene, two pairs of primers were designed and synthesized, which outer primers amplified fragment length was 297 bp,and inner primers amplified fragment length was 212 bp. RT-nested PCR methods had been established through optimization of the RT-PCR reaction conditions, this method was more sensitive and reliable than ordinary RT-PCR, and could effectively reduce the false positive and pollution. It could be used for rapid diagnosis and epidemiological investigation of the PEDV.

Key words: PEDV; N gene; RT-nested PCR; detection