Abstract: In order to explore the effect of dietary Huang Chong polypeptide (HCP) on fat and lipids metabolism in Wistar rats. 30 Wistar rats at 6 weeks were randomly allotted into 3 groups. We fed the basal diet which added 20% lard to the first group, and added 10% HCP, 10% fibre based on the first group diet to groups 2 and 3. The experiment period lasted for 8 months. Body weights were detected every weekend, and serum biochemical parameters and Leptin concentration were evaluated by the end of the experiment. The Real-time PCR method was used to dected the expression changes of Leptin mRNA in lipids. The results showed that the body weight of group 2 increased slower than groups 1 and 3 from 18 weeks, the fatty weight of epididymis were inhibited significantly in group 2(P＜0.01), viscera weights were no changed in groups 1, 2 and 3. The serum biochemical parameters of T-Bil, AST, ALT, ALP, BUN and Na⁺ had significant difference(P＜0.05,P＜0.01). The Leptin concentration of groups 2 and 3 were significantly lower than group 1 in serum and cerebrospinal fluid (CSF) (P＜0.01). The Leptin mRNA expression of group 2 was significantly higher than group 1(P＜0.05). The results indicated that Huang Chong polypeptide could inhibit and control fatty in rats.

Key words: Huang Chong polypeptide; Leptin; expression; fat