Abstract: According to the bovine keratin 14 (K14) gene sequence designed the PCR primers,and acquired the 2834 bp gene sequence(GenBank accession:JQ063036) by the DNA of Inner Mongolia Cashmere goat. This region included 2631 bp promoter region and 203 bp the first exon of structural gene of goat K14 gene. Sequence aligenment and analysis,and study the polymorphism of K14 promoter. It was found that goat’s and human’s structure of K14 promoter were different,both only having three common transcription factor binding sites such as Sox-5,GATA-1,GATA-1/2 binding sites. However,with the cattle’s had a higher similarity,containing the Sox-5,SRY,CdxA,MyoD,MZF1,GATA-1/3,Nkx-2 binding sites etc.7 common transcription factor binding sites. And it was found that goat K14 promoter region had five unique transcription factor binding sites,such as deltaE,GATA-2,GATA-1,Sp-1,AML-1a binding sites. In addition,the single nucleotide polymorphism (SNP) of goat K14 promoter was investigated,including four SNPs. Among these two novel SNPs were located in Nkx-2 binding site(-2004—-1996 bp) or near the several bases of Nkx-2 binding site Nkx-2(-1590—-1583 bp).

Key words: goat; K14 gene promoter; transcription factor; polymorphism