Abstract: The experimental material used Yunling goat oocytes from slaughterhouse,to study the effect of the oocytes vitrification. Using 20% EG+20% DMSO as a frozen solution and the cryloop for carrier to vitrify the GV and MⅡ stage oocytes in 20 s and 40 s vitrification time. The results showed that,GV oocytes morphologically normal rate,maturation rate and cleavage rate were very low. After maturation culture,the maturation rate and cleavage rate of the vitrification groups was significantly lower than the control group (P<0.01). MⅡ oocytes frozen was better,the rate of normal morphology of the toxicity test group and vitrification group were 91.1% and 83.3%,was significantly higher than the GV stage. After parthenogenetic activation,the cleavage rate,the toxicity test group and control group,were no significant differences(P>0.05). The vitrification group cleavage was significantly lower than the control group(P<0.05). The GV and MⅡ oocytes in each group were not significantly different in 20 s and 40 s vitrification time. According to the results it was the best to vitrify MⅡ oocytes in the cryopreservation,in order to improve the cleavage rate and blastocyst rate. The oocytes vitrification time did not affect oocyte viability and developmental potential in the 40 s inside.

Key words: goat; oocyte; vitrification; in vitro culture