3株柔嫩艾美耳球虫28SrRNA基因部分序列的扩增与分析

Abstract

Abstract: The partial 28S ribosomal RNA sequences of three different Eimeria tellena strains isolated from Guangdong province were amplified by polymerase chain reaction (PCR), and compared with relevant sequences of E.tellena, E. acervulina, Sarcocystis muris, T.gondii available in GenBank^TM. The results showed that the partial 28S rRNA sequence of three E.tellena strains was 1172 bp in length with no nucleotide difference in each other, and only one base difference found with E.tellena in GenBank^TM, revealing highly conservative within E.tellena. There were distinct sequence differences found comparing with E.acervulina, S.muris and T.gondii, which demonstrated 28S rRNA could be used as a genetic marker for the population genetic studies on different species of Eimeria and other apicomplexan parasites.

Key words: Eimeria tellena; 28S rRNA; PCR; sequence analysis

CLC Number:

S852.72

HOU Jie, LIN Rui-qing, LIU Li-dan, CHEN Jun-jia, WENG Ya-biao. PCR Amplification and Sequence Analysis of Partial 28S rRNA Sequence of three Eimeria tellena Strains[J]. , 2012, 39(7): 43-46.

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PCR Amplification and Sequence Analysis of Partial 28S rRNA Sequence of three Eimeria tellena Strains

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