猪催乳素受体基因第8内含子多态性及其对产仔性能的影响

Abstract

Abstract: The objective of this study was to detect the polymorphism of porcine prolactin receptor (PRLR) gene and analyze its association with litter size in swine, and to provide the basic knowledge for using PRLR gene as a genetic marker in marker assisted selection (MAS) for improving the reproduction performance in pig production. Polymorphisms of intron 8 of PRLR gene on 364 pigs from 6 breeds including Large White, Landrace, Duroc, Mashen pig, Shanxi Black pig and Shanxi White pig were tested by PCR-SSCP. Three alleles of A, B, C and six genotypes of AA, AB, AC, BB, BC and CC, were observed in examined populations. The A allele frequency was greater than those of B and C in Duroc (0.8276), Landrance (0.5439), Shanxi Black pig (0.5345), and Shanxi White pig (0.7669), whereas the B allele was superior to A and C in Large White (0.6894) and Mashen pig (0.7000). The C allele was in low frequency, ranged from 0.0379 to 0.1385, and only present in Large White, Shanxi Black pig and Shanxi White pig. Statistical analysis showed that PRLR genotype was significantly associated with total number born (TNB) (P<0.05) and was not associated with number born alive (NBA) at first parity. The TNB and NBA of heterozygote AC was significantly different from that of BC, whereas not significantly different from those of AA, AB and BB. The sows with A allele seems to had bigger TNB and NBA than those without allele A, which implied that the A allele was the favorable allele for improving sow’s litter size. The results of this work may provide the potential marker for the application in MAS for improving litter size in pig breeding and production.

Key words: pig; litter size; prolactin receptor gene; polymorphism; PCR-SSCP

CLC Number:

S813.3

SHI Jian-zhong, LI Bu-gao, CAO Guo-qing. Detection of the Polymorphism within Intron 8 of Prolactin Receptor Gene and its Association with Litter Size in Swine[J]. , 2012, 39(7): 179-183.

References

1 牟玉莲, 储明星, 孙少华, 等. 绵羊催乳素受体基因PCR-SSCP分析[J]. 畜牧兽医学报, 2006, 37(10):956~960.
2 张佳兰, 昝林森, 申光磊. 牛催乳素受体(PRLR)基因HinfⅠ多态性与奶牛生产性能相关性研究[J]. 畜牧兽医学报, 2007, 38(9): 888~892.
3 张淑君, 熊远著, 邓昌彦, 等. 母猪生殖器官大小和产仔数的分子遗传基础[J]. 遗传学报, 2002, 29(12): 1073~1077.
4 张跟喜, 储明星, 王金玉, 等. 催乳素受体基因外显子10多态性及其与济宁青山羊高繁殖力关系的研究[J]. 遗传, 2007, 29(3): 329~336.
5 洪坤月, 汪峰, 虞德兵, 等. 太湖鸡PRL、PRLR和FSHβ基因多态与前期产蛋性状关系研究[J]. 西北农业学报, 2007, 16(5): 11~14.
6 徐宁迎, 章胜乔, 彭淑红. 金华猪3个繁殖性状主基因的分布及其效应的研究[J]. 遗传学报, 2003,30(12): 1090~1096.
7 曹果清,李根银,王晓京,等. 猪催乳素受体基因第8内含子多态性检测及效应分析[J]. 中国畜牧兽医,2009,36(5):102~106.
8 曹果清, 李步高, 刘建华, 等. 猪催乳素受体(PRLR)基因AluⅠ多态性与产仔数关系的研究[J].山西农业大学学报, 2004a, 24(3): 253~255.
9 曹果清. 猪催乳素受体(PRLR)基因和促卵泡素β亚基(FSHβ)基因多态性研究.太谷:山西农业大学,2004b.
10 崔世泉, 李剑虹, 崔卫国, 等. 母猪哺乳初期的母性行为与催乳素受体基因多态性关系的初探[J]. 遗传, 2007, 29(1): 47~51.
11 Bachelot A,Binart N. Reproductive role of prolactin (review) [J]. Reproduction, 2007, 133: 361~369.
12 Horseman N D, Zhao W, Montecino-Rodriguez E, et al. Defective mammopoiesis, but normal hematopoiesis, in mice with a targeted disruption of the prolactin gene[J]. EMBO Journal, 1997, 16: 6926~6935.
13 Kelly P A, Binart N, Lucas B, et al. Implications of multiple phenotypes observed in prolactin receptor knockout mice[J]. Front Neuroendocrinol, 2001, 22:140~145.
14 Putnová L, Knoll A, Dvorak J,et al. A new HpaⅡ PCR-RFLP within the porcine prolactin receptor (PRLR) gene and study of its effect on litter size and number of teats[J]. J Anim Breed Genet, 2002, 119: 57~63.
15 Rothschild M F, Vincent A L, Tuggle C K, et al. A mutation in the prolactin receptor gene is associated with increased litter size in pigs[J]. Anim Genet, 1998, 29 (Suppl.1):69.
16 Terman A. Effect of the polymorphism of prolactin receptor (PRLR) and leptin (LEP) genes on litter size in polish pigs[J]. J Anim Breed Genet, 2005, 122: 400~404.
17 Tomás A, Casellas J, Ramiez O, et al. High amino acid variation in the intracellular domain of the pig prolactin receptor (PRLR) and its relation to ovulation rate and piglet survival traits[J]. J Anim Sci, 2006, 84: 1991~1998.
18 van Rens B T, van der Lende T. Litter size and piglet traits of gilts with different prolactin receptor genotypes[J]. Theriogenology, 2002, 57(2): 883~893.
19 van Rens B T, Evans G J, van der Lende T. Components of litter size in gilts with different prolactin receptor genotypes[J]. Theriogenology, 2003, 59: 915~926.
20 Viengchareun S, Serve N, Fève B, et al. Prolactin receptor signaling is essential for perinatal brown adipocyte function: a role for insulin-like growth factor-2[J]. PloS One, 2008, 2: e1535.
21 Vincent A L, Wang L, Tuggle C K, et al. Prolactin receptor maps to pig chromosome 16[J]. Mamm Genome, 1997, 8: 793~794.
22 Vincent A L, Evans G, Short T, et al. The prolactin receptor is associated with increased litter size in pigs. In: Proc 6th World Congr Genet App Livest Prod, 1998, 15~18.
23 Wells J A, Devos A M. Hematopoietic receptor complexes [J]. Annu Rev Biochem, 1996, 65: 609~634.

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Detection of the Polymorphism within Intron 8 of Prolactin Receptor Gene and its Association with Litter Size in Swine

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