›› 2012, Vol. 39 ›› Issue (5): 79-81.

• 生物技术 • Previous Articles     Next Articles

Establishment of RT-PCR for Detection of Porcine Epidemic Diarrhea Virus

LUO Yong-zhen   

  1. The Veterinary Station of Chengguan Town, Huangyuan City in Qinghai Province, Huangyuan 812100, China
  • Received:2011-10-04 Revised:1900-01-01 Online:2012-05-20 Published:2012-05-20

Abstract: The article acts according to nucleocapsid protein gene sequence on GenBank and designs one pair of primers which could amplify 245 bp fragment. A series of test results indicated that this method has the very strong specificity and the very high sensitivity, may take porcine epidemic diarrhea virus the clinical fast diagnosis one method.

Key words: porcine epidemic diarrhea virus; RT-PCR; detection 书 讯 2010; 2008年合订本(每册128元); 并有少量2004; 2005年合订本(每册70元); 2003年合订本(每册40元)。如有订购者; 请汇款到:100193中国农业科学院北京畜牧兽医研究所 《中国畜牧兽医》编辑部收。

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