牦牛病毒性黏膜病病毒P125基因的克隆及序列分析

Abstract

Abstract: According to the sequence data of BVDV strain published by GenBank, five set of primers were designed and used to amplify P125 gene of BVDV strain yak by the method of PCR. A specific 3475 bp DNA segment was amplified, which was cloned into pMD19-T vector. The positive recombinant clone was identified by plasmid PCR and restriction enzyme digestion. Compared with BLAST, the P125 gene of BVDV strain yak exhibited the highest homology with strain NADL, but they only shared 63.4%, showing that the P125 gene of BVDV strain yak had great variation. This might be the virus to adapt to yak and the ecological environment of the plateau,or the virus might also have an independent source of genetic.

Key words: yak; bovine viral diarrhea virus; P125 gene; cloning; bioinformatics analysis

CLC Number:

LIU Ya-gang, SUN Kai, WANG Yan, WANG Pan-pan, HU Bing-feng, WANG Wen-bo. Cloning and Bioinformatics Analysis of the P125 Gene of Bovine Viral Diarrhea Virus Strain Yak[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(12): 27-31.

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Cloning and Bioinformatics Analysis of the P125 Gene of Bovine Viral Diarrhea Virus Strain Yak

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