Abstract: According to the gene sequences in GenBank of Salmonella enteritidis from chickens, one pair of specific primers was designed for amplifying the specific fragments of ompA gene. The ompA gene was amplified from the isolated Guizhou strain of SE by PCR, and subcloned the sequence into the prokaryotic expression vector pET-32a(+).The recombinant plasmid pET-32a-ompA was transformed into E.coli BL21(DE3) and expressed under the induction of IPTG. The result of SDS-PAGE showed that the expressed protein was about 55 ku, and the recombinant protein was in form of inclusion bodies. Western blotting analysis indicated that this protein had immunological activity.

Key words: chickens; Salmonella enteritidis; ompA gene; prokaryotic expression