猪繁殖与呼吸综合征病毒GZ-KY1株的分离及ORF5基因的克隆分析

›› 2012, Vol. 39 ›› Issue (12): 14-16.

Previous Articles Next Articles

Isolation and Clone Analysis of ORF5 Gene of PRRSV GZ-KY1 Strain

LIU Zhi-jie¹, ZENG Zhi-yong^1,2, ZHOU Li¹, LIANG Hai-ying³

1. College of Animal Science,Guizhou University,Guiyang 550025,China;
2. Guizhou Province Key Laboratory of Animal Epidemic Disease and Veterinary Public Health,Guiyang 550025,China;
3. Teaching Experiment Farms,Guizhou University,Guiyang 550025,China

Received:2012-06-08 Online:2012-12-20 Published:2012-12-19

Abstract

Abstract: In this experiment, a strain of porcine reproductive and respiratory syndrome virus (PRRSV) was isolated in PK-15 cells by using synchronous inoculation method, named GY-KY1 strain, and the ORF5 gene was cloned. The results demonstrated that GY-KY1 strain was American type and the ORF5 gene was 603 bp. The amino acid consistency of GY-KY1 strain with domestic virulent strain than the attenuated strain, and had the farest genetic relationship with the classic American strains and domestic attenuated strain.

Key words: PRRSV; PK-15; Marc-145; isolation and identification

CLC Number:

Q78

LIU Zhi-jie, ZENG Zhi-yong, ZHOU Li, LIANG Hai-ying. Isolation and Clone Analysis of ORF5 Gene of PRRSV GZ-KY1 Strain[J]. , 2012, 39(12): 14-16.

[1]	KANG Pu, REN Zhaowen, CAI Rujian, LYU Zongji, HUANG Yuhao, WANG Xiaohu. Variation Regularity and Transmission of PRRSV-2 Lineage 8 Strains in Guangdong and Surrounding Provinces [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2001-2011.
[2]	CHEN Tianbao, WANG Yani, LIU Baoling, WANG Gang, WANG Xiaohu, LIU Xuhui, CAI Rujian, HE Dongsheng. Research Progress on the Anti-Porcine Reproductive and Respiratory Syndrome Virus Effect and Mechanism of Traditional Chinese Medicine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2114-2129.
[3]	LI Huawei, GUO Kewei, WANG Xuying, ZHANG Xiaoyu, LU Ziwei, LI Xinfeng, MA Hui, HOU Wenjing. Eukaryotic Expression and Subcellular Localization of Nonstructural Protein 7 of Porcine Reproductive and Respiratory Syndrome Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1160-1168.
[4]	CHEN Yanxi, WANG Chen, LUO Yuan, ZHOU Yuancheng, XU Zhiwen, PENG Yuanyi, SONG Zhenhui, LIU Xiao. circRNA Expression Profile Analysis of PK-15 Cells Response to Seneca Virus A Infection [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 280-289.
[5]	ZHOU Wei, LIU Xuping, ZHANG Yanmin, PENG Wenjuan, ZHAO Liang, TAN Wensong. Serum-free Suspension Culture of PK-15 Cells and Its Effect on the Proliferation of Porcine Circovirus Type 2 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2397-2405.
[6]	XIE Sihao, GOU Hongchao, BIAN Zhibiao, LI Bin, CAI Rujian, ZANG Yingan, LI Chunling. Effects of MLKL Gene Knockout on Replication of Pseudorabies Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1934-1941.
[7]	LIU Qingqing, WU Peng, LI Peidong, ZHANG Jiangwei, CHEN Chuangfu, XIAO Chencheng. Construction of Recombinant Adenovirus Expressing Porcine Reproductive and Respiratory Syndrome Virus N Protein and Its Immunogenicity Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1024-1031.
[8]	WANG Zhaoli, YANG Siyu, WU Yanmei, SONG Xiaoxiao, MU Xiang, ZHANG Tao. Effects of PRRSV-LPS Treatment on Neutrophil Adhesion to Porcine Pulmonary Microvascular Endothelial Cells [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 471-479.
[9]	SUN Lu, ZHANG Jing, YIN Miao, CHENG Yaodie, DAI Kun, WU Qian, ZHAO Hong, CHEN Xiwen. Study on the Antibacterial and Anti-PRRSV Activities of Four Heat Clearing and Detoxifying Compound Chinese Herbal Medicine in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 746-754.
[10]	CHU Pinpin, JIANG Zhiyong, LI Chunling, GOU Hongchao, LI Yan, CAI Rujian. Antiviral Effect of Silver Nanoparticles on Porcine Reproductive and Respiratory Syndrome Virus in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4356-4363.
[11]	DENG Jiping, CHEN Zhilong, GUO Songyangnian, ZHANG Kun, XU Daojun, YANG Qing, YUAN Anwen. Genetic Evolution Analysis of ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus Isolated from Hunan Province [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3795-3802.
[12]	LIU Hanyang, ZHANG Yi, XIAO Ran, CHEN Bin, WU Yongsheng, SU Zhipeng, LI Yupeng, PEI Chaoxin, XU Zhenying, ZHU Jiawen. Molecular Epidemiological Investigation on ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus in Sichuan in 2018 [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1172-1182.
[13]	ZHANG Zonghui, WANG Xinyi, CAO Haihong, HU Xiao, WANG Baobao, GAO Fengshan. A Study on the Expression of β2m Gene in Swine Kidney Cells [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 372-380.
[14]	ZHOU Shumian, CAO Mixia, HU Tingjun. A Study on Effect of Anti-PRRSV of n-butanol Part of Flavonoids from Polygonum hydropiper L. in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 605-611.
[15]	ZHANG Xiyi, WEN Guilan, GUAN Guodan, CHEN Guang, TIAN Lang, YANG Baiqi. Cloning and Genetic Variation Analysis of NSP2 Gene of Porcine Reproductive and Respiratory Syndrome Virus Guizhou Strain GZ-R [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3659-3666.

Isolation and Clone Analysis of ORF5 Gene of PRRSV GZ-KY1 Strain

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments