牛PU.1基因真核表达载体的构建、表达及鉴定

Abstract

Abstract: Total RNA was extracted from bovine,the cDNA encoding PU.1 protein was cloned using reverse transcription polymerase chain reaction (RT-PCR). The PU.1 gene was subcloned into pIRES2 DsRed-Express2 vector and then the recombinant plasmids were transferred into H293T cells and bovine fetal fibroblasts cells by the method of lipofectaming. The results showed that we had successfully constructed the expression plasmid of PU.1 gene by fluorescence observation and identification of Western blotting,which layed the foundation for the differentiation of bovine blood monocytes and breeding for disease resistance in cattle.

Key words: cattle; PU.1 gene; monocytes

CLC Number:

LIU Li, ZHANG Lu-pei, GAO Xue, GAO Hui-jiang, LI Jun-ya, XU Shang-zhong. Construction,Expression and Identification of Eukaryotic Expression Vector of Bovine PU.1 Gene[J]. , 2012, 39(11): 69-72.

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Construction,Expression and Identification of Eukaryotic Expression Vector of Bovine PU.1 Gene

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