冷冻速率和解冻温度对猪精液冷冻效果的影响

Abstract

Abstract: For improving the freezing and de-freezing methods for boar semen further, it was aimed to investigate the effect of different freezing frequency(-100 ℃ 10 min,-120 ℃ 10 min,-140 ℃ 10 min) and thawing temperature(37 ℃ 30 s,45 ℃ 30 s,52 ℃ 30 s,60 ℃ 30 s) on cryopreservation of boar semen. The results showed that the sperm motility, plasma integrity, normal acrosome rate by freezing treatment with -120 ℃ 10 min, were all significantly higher than those of the other three groups respectively(P＜0.05). The sperm motility, plasma integrity, normal acrosome rate by thawing treatment with 37 ℃ 30 s, were not significantly higher than those of the other three groups respectively(P＞0.05), but the difference of the sperm abnormality in the treatment with 60 ℃ 30 s was significant(P＜0.05), but with 45 ℃ 30 s and 52 ℃ 30 s were not significant(P＞0.05). Therefore, it could be concluded that the combination of freezing by -120 ℃ 10 min and thawing by 37 ℃ 30 s were appropriate to frozen-thawed 0.25 mL pipettes of boar semen.

Key words: boar; cryopreservation; dtraw; freezing frequency; artificial insemination

CLC Number:

S828.3⁺4

LIANG Hong-bin, YAO Xue-jun, LI Wei-hua, SHAO Hui, ZHU Jin-qing, WU Chun-hong, NI He-min, LIU Yun-hai, GUO Yong. Effect of Different Freezing Frequency and Thawing Temperature on Cryopreservation of Boar Semen[J]. , 2012, 39(11): 157-160.

References

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Effect of Different Freezing Frequency and Thawing Temperature on Cryopreservation of Boar Semen

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