Abstract: In order to improve the frozen porcine sperms motility and fertilizing ability after thawed, sperms were extended in basic cryoprotection extender with 0, 10%, 20%, 30% seminal plasma respectively before freezing. Sperm motility, membrane integrity, fertilization rate and blastocyst rate of IVF embryos and artificial insemination results were chosen as criteria for assessing the sperm quality. The results showed that the motility, membrane integrity of sperms extended in cryoprotection extender containing 10% seminal plasma were improved significantly (P<0.05) after thawed compared with the control group. The blastocyst rate of IVF embryos derived from these sperms was also increased significantly. Artificial insemination with the thawed sperms from the group extended in cryoprotection extender containing 10% seminal plasma resulted in a significantly lower pregnancy rates, litter size and number of live littermate compared with fresh sperm insemination(P<0.05). These results indicated that cryoprotectant with 10% seminal plasma could increase the motility of thawed porcine sperm after cryopreservation and the blastocyst rate after IVF, but for artificial insemination it was still need to be improved.

Key words: porcine sperm; seminal plasma; cryopreservation; IVF; artificial insemination