云南鸭疫里默氏杆菌外膜蛋白A 及16S rRNA序列分析

Abstract

Abstract: To analysis the OmpA and 16S rRNA gene of Riemerella anatipestifer isolated strains in Yunnan province and explore the correlationship between OmpA and 16S rRNA gene on phylogenetic trees, OmpA and 16S rRNA gene was amplified respectively by PCR to construct the phylogenetic trees. Results analysis showed that the OmpA gene of 18 Yunnan isolated RA strains were situated in two groups and their homology were 86% to 99.2% and 92.6% to 100% respectively, the 16S rRNA gene of 18 Yunnan isolated RA strains were situated in one group and their homology was 96.1% to 100%.It’s worth noting that the RA-1,RA-2,RA-1 and RA-39 isolated strains belonged to the same subgroup on both the OmpA phylogenetic tree and 16S rRNA phylogenetic tree with the higher homology 97.3% to 99.2% and 99.6% to 100% respectively, the two phylogenetic trees showed a significant correlation within RA-1,RA-2,RA-1 and RA-39 isolated strains and showed no correlation within the other 14 isolated strains.

Key words: Riemerella anatipestifer; OmpA gene; 16S rRNA gene; sequence analysis

CLC Number:

S855.1⁺2

LI Fu-xiang, WANG Chuan-yu, CHANG Zhi-shun, YANG Bin, LI Hua-chun. The Sequence Analysis of OmpA and 16S rRNA Gene of Riemerella anatipestifer Isolated Strains in Yunnan Province[J]. , 2012, 39(10): 61-65.

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The Sequence Analysis of OmpA and 16S rRNA Gene of Riemerella anatipestifer Isolated Strains in Yunnan Province

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