猪A型流感病毒RT-PCR的建立及应用

›› 2011, Vol. 38 ›› Issue (8): 97-99.

• 生物技术 • Previous Articles Next Articles

Establishment of RT-PCR for Detection of Influenza A Virus among Swine Herds and its Application

ZHAO Pu^1,2, ZHAO Kun^1,2, ZHENG Yu-shu^1,2, LI Ren-feng¹, HU Jian-he^1,2, WANG San-hu^1,2, LIU Xing-you^1,2, JIA Bei-bei¹

1. Department of Animal Science and Technology,Henan Institute of Science and Technology,Xinxiang 453003,China;2. Henan Engineering Research Center of Animal Disease and Residue Prevention and Control,Xinxiang 453003,China

Received:1900-01-01 Revised:2011-03-17 Online:2011-08-20 Published:2011-08-20

Abstract

Abstract: According to the conserved sequence in the matrix (M) gene of influenza A virus published in GenBank,a pair of primers for amplification influenza A virus specified fragment were designed and a RT-PCR assay for detection influenza A virus among pigs was established. The results showed that the RT-PCR was sensitive and specific,and useful to detect the clinical samples. It is the foundation for clinical diagnosis and epidemiologic survey of influenza A virus circulating in swine herds.

Key words: swine herds; influenza A virus; RT-PCR; clinical diagnosis; epidemiologic survey

CLC Number:

S854.4

ZHAO Pu;ZHAO Kun;ZHENG Yu-shu;LI Ren-feng;HU Jian-he;WANG San-hu;LIU Xing-you;JIA Bei-bei. Establishment of RT-PCR for Detection of Influenza A Virus among Swine Herds and its Application[J]. , 2011, 38(8): 97-99.

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Establishment of RT-PCR for Detection of Influenza A Virus among Swine Herds and its Application

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