›› 2011, Vol. 38 ›› Issue (6): 175-178.

• 疾病防治 • Previous Articles     Next Articles

Isolation and Identification of an Avian Influenza Virus of Subtype H9

ZHANG Fa-ming, ZHENG Jie, WANG Wen-quan, CHEN Ling, XING Xue-lian, ZHANG Hong, LI Jing, JIA Xiu-zhen, CHE Jing, ZHANG Hong   

  1. Beijing Veterinary Biological Products Factory,Beijing 102600, China
  • Received:2010-12-06 Revised:1900-01-01 Online:2011-06-20 Published:2011-06-20

Abstract: April 2010,there was avian disease occurred to local chicken breeding farm in Anhui province,characterized by severe breathing difficulties and death suddenly,in this research,kidney and lung samples of chicken were made to suspensions which inoculated into the allantoic cavity of SPF embryo,then the research group obtained a purified virus by the method of limiting dilution method. The research result of HA/HI,molecular biology and animal regression testing suggests that the disease was H9 subtype of avian influenza.

Key words: broiler; avian influenza virus H9 subtype; separation; identification

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