Abstract: A pair of special primers was designed according to the sequence of IFN-γ in GenBank. Total RNA was extracted from cow spleen lymphocyte,IFN-γ gene was amplified by the reverses transcription polymerase chain reaction. After linked with pMD18-T vector,it was digested by enzyme,PCR and gene sequencing were carried. And this fragment was cloned into the multiple cloning sites of pQE-30 vector. The recombinant plasmid was designated pQE30-IFN-γ and was transformed into E.coli x-LI Blue competent cells. Expression of the recombinant protein was induced by IPTG under 37 ℃ condition. The length of PCR product of recombinant plasmid was 510 bp,the size of recombinant protein was 22 ku. The results of Western blotting showed that it could be recognized by anti-His tagged mouse monoclonal antibody. These results were benefit to research for function of IFN-γ.

Key words: IFN; gene; clone; expression 