›› 2011, Vol. 38 ›› Issue (3): 51-54.

• 生理生化 • Previous Articles     Next Articles

Adiponectin Effect of ADPN mRNA，Hormone-sensitive Lipase mRNA Expression on Primary Cultured Bovine Adipocytes

YU Guo-jian1，LIU Guo-wen1，LI Xiao-bing1，WANG Zhe1，LI Peng1,3，HUANG Hai-long1,2，ZHANG Yi1,3   

  1. 1.College of Animal Science and Veterinary Medicine，Jilin University，Changchun 130062，China；2.College of Animal Science and Technology，Jilin Agricultural University，Changchun 130118，China；3.College of Animal Science and Veterinary Medicine，Shenyang Agricultural University，Shenyang 110161,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-03-20 Published:2011-03-20

Abstract: This experiement on adipocyte of calf was adopt to make primary culture，select monolayer growth well adipocyte of calf，then add concentration of the additives was as ADPN: 0,2.5,5,15,30,50,100 μg/mL, after 12 h cultivation，abstract total RNA and test the effect of ADPN on the fat cell of ADPN mRNA and HSL mRNA expression by FQ-PCR method. With increasing the addition of ADPN, expression level of ADPN mRNA decreased; the expression level of HSL mRNA in fat cell was highest when the concentration of ADPN was 15 μg/mL in cell culture media，then decreased. The results showed as follows: the ADPN mRNA expression was regulated by negative regulator itself in adipocyte culture in vitro, but the appropriate amount concentration of ADPN could stimulate adipocyte HSL mRNA expression.

Key words: adiponectin; adipocytes; ADPN mRNA; HSL mRNA

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