猪瘟石门系强毒株SYBR Green-Ⅰ染料法实时荧光定量PCR方法的建立

›› 2011, Vol. 38 ›› Issue (3): 104-107.

• 生物技术 • Previous Articles Next Articles

Development of a Real-time Flourescent Quantitative PCR for Detection of Classical Swine Fever Virus Shimen Strain

WANG Wei1, ZOU Yue-hong2, LI Xue-song3, CHEN Xin3, LANG Yue-kun3, TIAN Hua-bin3, FU Fang3, LI Xi3, LI Ji-lin1

（1.College of Life Science and Technology, Harbin Normal University, Harbin 150001, China; 2.Heilongjiang University, Harbin 150001, China; 3.State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China）

Received:1900-01-01 Revised:1900-01-01 Online:2011-03-20 Published:2011-03-20
Contact: LI Ji-lin；LI Xi

Abstract

Abstract: In order to be able to fast and accurate diagnosis of classical swine fever virus strain Shimen based on the alignment of genomic sequences of 23 strains of Shimen available in GenBank, one pair of differential primers were designed by DNASIS software.SYBR Green-Ⅰ real-time PCR for detection of classical swine fever virus strain Shimen was established. The sensitivity was 100 copies/μL. The assay is highly specific and showed no cross reactivity against PRRSV, PCV2, PRV, BVDV-1 and C-strain. Inter-assay and intra-assay variables of CV were less than 0.7%. This method was highly sensitive specific and rapid. This assay could provide a powerful tool for quantification of Shimen-strain, and could be further developed into a commercial kit for rapid detection of Shimen-strain.

Key words: CSFV; Shimen-strain; SYBR Green-Ⅰ real-time PCR

CLC Number:

WANG Wei;ZOU Yue-hong;LI Xue-song;CHEN Xin;LANG Yue-kun;TIAN Hua-bin;FU Fang;LI Xi;LI Ji-lin. Development of a Real-time Flourescent Quantitative PCR for Detection of Classical Swine Fever Virus Shimen Strain[J]. , 2011, 38(3): 104-107.

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Development of a Real-time Flourescent Quantitative PCR for Detection of Classical Swine Fever Virus Shimen Strain

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