猪繁殖与呼吸综合征病毒2b基因的克隆和原核表达

China Animal Husbandry & Veterinary Medicine ›› 2011, Vol. 38 ›› Issue (12): 67-70.

• 生物技术 • Previous Articles Next Articles

Cloning and Expression of 2b Protein Gene of Porcine Reproductive and Respiratory Syndrome Virus

HE Bin^1,2,3,4, LI Yong^1,2,3,4, ZHAO Jian-zeng¹, GAO Ying-jie¹, XIA Zhi-ping², WANG Ze-yan¹, LIU Wen¹, ZHANG Ning¹, QU Xue-qi¹, BAI Xue¹, MEI Lin¹, MA De-hui³

1. College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2. Military Veterinary Institute,Academy of Military Medicine Science of PLA,Changchun 130062,China;3. Inner Mongolia Uneversity for the Nationalities,Tongliao 028042,China;4. Veterinary Prevention and Treatment Station of Ruicheng,Shanxi Province,Ruicheng 044600,China

Received:2011-04-19 Revised:1900-01-01 Online:2011-12-20 Published:2011-12-20

Abstract

Abstract: A pair of specific primers of porcine reproductive and respiratory syndrome virus (PRRSV) 2b were designed and synthesized according to the published gene sequence of PRRSV from GenBank,and 2b gene of PRRSV was obtained by RT-PCR method. The RT-PCR product was cloned into pMD18-T vector and sequenced. After the positive gene was cloned into the prokaryotic expression vector and designated pGEX-4T-2b,the recombinant plasmid was transformed into host cell BL21(DE3) and induced by IPTG. SDS-PAGE and Western blotting test confirmed that the expressed fusion protein is about 35 ku,which had immunogenically reactive activity.

Key words: porcine reproductive and respiratory syndrome virus; 2b protein; clone; expression

CLC Number:

HE Bin;;LI Yong;;ZHAO Jian-zeng;GAO Ying-jie;XIA Zhi-ping; WANG Ze-yan;LIU Wen;ZHANG Ning;QU Xue-qi;BAI Xue;MEI Lin;MA De-hui. Cloning and Expression of 2b Protein Gene of Porcine Reproductive and Respiratory Syndrome Virus[J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(12): 67-70.

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Cloning and Expression of 2b Protein Gene of Porcine Reproductive and Respiratory Syndrome Virus

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