猪繁殖与呼吸综合征病毒2b基因的克隆和原核表达

›› 2011, Vol. 38 ›› Issue (12): 67-70.

• 生物技术 • Previous Articles Next Articles

Cloning and Expression of 2b Protein Gene of Porcine Reproductive and Respiratory Syndrome Virus

HE Bin^1,2,3,4, LI Yong^1,2,3,4, ZHAO Jian-zeng¹, GAO Ying-jie¹, XIA Zhi-ping², WANG Ze-yan¹, LIU Wen¹, ZHANG Ning¹, QU Xue-qi¹, BAI Xue¹, MEI Lin¹, MA De-hui³

1. College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2. Military Veterinary Institute,Academy of Military Medicine Science of PLA,Changchun 130062,China;3. Inner Mongolia Uneversity for the Nationalities,Tongliao 028042,China;4. Veterinary Prevention and Treatment Station of Ruicheng,Shanxi Province,Ruicheng 044600,China

Received:2011-04-19 Revised:1900-01-01 Online:2011-12-20 Published:2011-12-20

Abstract

Abstract: A pair of specific primers of porcine reproductive and respiratory syndrome virus (PRRSV) 2b were designed and synthesized according to the published gene sequence of PRRSV from GenBank,and 2b gene of PRRSV was obtained by RT-PCR method. The RT-PCR product was cloned into pMD18-T vector and sequenced. After the positive gene was cloned into the prokaryotic expression vector and designated pGEX-4T-2b,the recombinant plasmid was transformed into host cell BL21(DE3) and induced by IPTG. SDS-PAGE and Western blotting test confirmed that the expressed fusion protein is about 35 ku,which had immunogenically reactive activity.

Key words: porcine reproductive and respiratory syndrome virus; 2b protein; clone; expression

CLC Number:

HE Bin;;LI Yong;;ZHAO Jian-zeng;GAO Ying-jie;XIA Zhi-ping; WANG Ze-yan;LIU Wen;ZHANG Ning;QU Xue-qi;BAI Xue;MEI Lin;MA De-hui. Cloning and Expression of 2b Protein Gene of Porcine Reproductive and Respiratory Syndrome Virus[J]. , 2011, 38(12): 67-70.

[1]	WANG Hui, FENG Baoliang, XIANG Guangming, HUANG Lei, LIU Zhiguo, LI Kui, MU Yulian. Construction of iPAMs with CD163 Monoallelic Expression and Characterization of Their Mediating PRRSV Infection [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2617-2628.
[2]	LIU Jialong, ZHANG Yiwen, ZHANG Yueran, SUN Qijuan, CHEN Jian, HE Lei, JIA Yanyan, DING Ke, YU Zuhua. Cloning,Prokaryotic Expression and Bioinformatics Analysis of ELAVL1 Gene in Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1807-1817.
[3]	KANG Pu, REN Zhaowen, CAI Rujian, LYU Zongji, HUANG Yuhao, WANG Xiaohu. Variation Regularity and Transmission of PRRSV-2 Lineage 8 Strains in Guangdong and Surrounding Provinces [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2001-2011.
[4]	CHEN Tianbao, WANG Yani, LIU Baoling, WANG Gang, WANG Xiaohu, LIU Xuhui, CAI Rujian, HE Dongsheng. Research Progress on the Anti-Porcine Reproductive and Respiratory Syndrome Virus Effect and Mechanism of Traditional Chinese Medicine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2114-2129.
[5]	LI Huawei, GUO Kewei, WANG Xuying, ZHANG Xiaoyu, LU Ziwei, LI Xinfeng, MA Hui, HOU Wenjing. Eukaryotic Expression and Subcellular Localization of Nonstructural Protein 7 of Porcine Reproductive and Respiratory Syndrome Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1160-1168.
[6]	LYU Shuangfei, MA Xun, WANG Jing, KONG Cuilian, KOU Lijun, LIU Caixia, SHI Weidi, KANG Lichao, REN Huijie. Prokaryotic Expression and Immunoreactivity of PA-LF1 Fusion Protein of Bacillus anthracis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 674-683.
[7]	WANG Dan, ZHANG Xinyu, FU Jiaqi, JIA Qi, ZHANG Shuangshuang, CUI Chengdu, ZHANG Lichun, SUN Fuliang. Cloning,Bioinformatics Analysis and Tissue Expression of SMAD4 Gene in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 4783-4792.
[8]	GUO Chaohui, ZHANG Zihao, LI Haijie, ZHANG Meina, JIANG Xin, YU Guangqing, LI Ming, LIU Bianzhi. Cloning,Bioinformation and Function Analysis of RNF145 Gene in Cyprinus carpio [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 3882-3895.
[9]	YAN Pupu, LIU Xin, LIN Bing, LIU Jiali, XIA Jinjin, HUANG Yongxi, BAI Ruonan, LIU Guoping, YANG Xiaolin, GUO Liwei. Exploring of the Infection Characteristics of Porcine Reproductive and Respiratory Syndrome Virus and the Prevention and Control Mechanism of Yinhua Gancao Decoction Based on Bioinformatics [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(10): 4196-4209.
[10]	LIU Qingqing, WU Peng, LI Peidong, ZHANG Jiangwei, CHEN Chuangfu, XIAO Chencheng. Construction of Recombinant Adenovirus Expressing Porcine Reproductive and Respiratory Syndrome Virus N Protein and Its Immunogenicity Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1024-1031.
[11]	WANG Zhaoli, YANG Siyu, WU Yanmei, SONG Xiaoxiao, MU Xiang, ZHANG Tao. Effects of PRRSV-LPS Treatment on Neutrophil Adhesion to Porcine Pulmonary Microvascular Endothelial Cells [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 471-479.
[12]	SUN Lu, ZHANG Jing, YIN Miao, CHENG Yaodie, DAI Kun, WU Qian, ZHAO Hong, CHEN Xiwen. Study on the Antibacterial and Anti-PRRSV Activities of Four Heat Clearing and Detoxifying Compound Chinese Herbal Medicine in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 746-754.
[13]	CHU Pinpin, JIANG Zhiyong, LI Chunling, GOU Hongchao, LI Yan, CAI Rujian. Antiviral Effect of Silver Nanoparticles on Porcine Reproductive and Respiratory Syndrome Virus in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4356-4363.
[14]	WANG Miao, CHEN Xueyang, WANG Xingming, LIANG Xiongyan, YANG Yuying. Construction of Infectious Clones of Two ALV-K Recombinant Viruses and Virus Rescue [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3764-3770.
[15]	ZHANG Qi, CHEN Haotian, SHAN Jiabao, CHANG Rui, ZHANG Xue, WANG Yao, WANG Junwei, MA Bo. Cloning and Sequence Analysis of NS1 and VP1 Genes of Gosling Plague Virus from Jilin District [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1161-1169.

Cloning and Expression of 2b Protein Gene of Porcine Reproductive and Respiratory Syndrome Virus

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments