Abstract: Metabolomics/metabonomics s tudy of Sudan Ⅳ was depend on the eggs of Highland Brown layers with determination by Agilent 6530 liquid chromatography-quadrupole/time of flight mass spectrometer (LC-MS/Q-TOF), which was high resolution mass spectrmeter with electrospray ionization(+/-).The egg samples were obtained from hens which were fed Sudan Ⅳ(0,100,150,200 mg/kg)and were taken after 28 days-feeding.Then endogenous metabolites'information of eggs could be acquired by principal component analysis of statistical analysis software (mass profiler professional, MPP). Results showed that, this method could distinguish blank control group and the feeding of Sudan Ⅳ group clearly. Endogenous metabolites which have significantly differences (P<0.05, fold change≥2) had been preliminary identified by searching in database with 23000 metabolites.The results provided a basic work for further studies of metabolomics/metabonomics of Sudan Ⅳ.

Key words: Sudan Ⅳ; metabolomics/metabonomics; liquid chromatography-quadrupole/time of flight mass spectrometer (LC-MS/Q-TOF); electrospray ionization source; metabolite