China Animal Husbandry & Veterinary Medicine ›› 2011, Vol. 38 ›› Issue (11): 57-60.

• 生理生化 • Previous Articles     Next Articles

Effects of Different Species Bovine Sera on Proliferation and Differentiation in 3T3L1 Preadipocyte

FENG Li-ping1, SONG Zhong-feng1, SHI Qiong1, LI Wen-bo2,3, LUO Xiao-yu1   

  1. 1. College of Animal Science and Technology,China Agricultural University,Beijing 100193,China;2. Ningxia Zhongwei Goat Breeding Farm, Zhongwei 755000,China;3. Ningxia Hui Autonomous Region Animal Workstations, Yinchuan 750001,China
  • Received:2011-04-20 Revised:1900-01-01 Online:2011-11-20 Published:2011-11-20

Abstract: The purpose of this study was to know the effects of different species bovine sera on the proliferation and differentiation of 3T3-L1 preadipocytes. In order to provide the possible method of early selection for marbling,we use cell model in vitro culture to simulate the environment of bovine adipose tissue. Collected the bovine sera of different species from seventeen Qinchuan cattle and twenty-eight Qinchuan- Angus hybrid cattle. One part of sera were heat inactivated. The other were activated. The relatively cell number,which stands for proliferation of 3T3-L1 cells,was assayed by MTT colorimetry. Fat content and enzyme activities,which contain glycerol phosphate dehydrogenase (GPDH) and fatty acid synthetase (FAS),represented the differentiation. Oil red O staining was used to determine the fat content,and enzyme activities were determined by colorimetry after inducting adipogenesis. The results showed as follow. First,heat inactivated sera were quite significantly higher than activated ones on relatively cell number of 3T3-L1 cell at day 2 and 4,and it's the same results with fat content at day 2,4,6 and 8 (P＜0.01). Second,Qinchuan-Angus hybrid bovine sera were significantly higher than Qinchuan bovine sera on relatively cell number at day 2 and 4 in cell culture (P＜0.05). Qinchuan sera were significantly higher than hybrid sera on lipid content at day 8 (P＜0.05). However,there were no significant different at other days on cell number and lipid content (P＞0.05). Moreover,there were no significantly different between two species of cattle sera on GPDH and FAS activities in cell culture at day 8 (P＞0.05). Self-made bovine sera within heat inactivation promoted proliferation and differentiation of 3T3-L1 cell. Bovine sera is one of the factors that affect proliferation and differentiation of 3T3-L1 cell. Qinchuan- Angus hybrid bovine sera may be good for proliferation,wahile Qinchuan bovine sera may be good for differentiation. More studys are needed.

Key words: species; bovine sera; poliferation; differentiation; 3T3-L1 preadipocyte

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