China Animal Husbandry & Veterinary Medicine ›› 2011, Vol. 38 ›› Issue (11): 125-131.

• 遗传繁育 • Previous Articles     Next Articles

The Methylation Status of IGF2/H19 Imprinting Control Region in Cloned Pig

WU Zhi-qiang1,2,3, XIE Yi-ni1,2,3, ZHANG Ting-yu1,2, DAI Jian-jun1,2, WU Cai-feng1,2, MA Heng-dong3, ZHANG De-fu1,2   

  1. 1. College of Life and Basic Sciences,Sichuan Agricultural University,Ya'an 625014,China;2. Institute of Animal Science and Veterinary Medicine,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China;3. Division of Animal Genetic Engineering,Shanghai Municipal Key Laboratory of Agri-genetics and Breeding,Shanghai 201106,China
  • Received:2011-04-06 Revised:1900-01-01 Online:2011-11-20 Published:2011-11-20

Abstract: In order to make sure whether the DNA methylation reprogramming is efficient in SCNT animals,we analyzed the DNA methylation status of IGF2/H19 imprinting control region (DMR1,DMR2,DMR3) in heart,liver,spleen,lung and kidney of cloned pig using bisulfite sequencing analysis. The results demonstrated that the methylation level of DMR1 and DMR3 in dead cloned pig tissues were different from that of normal pig,but there was no significant differences among them (P＞0.05);The DMR2 showed hypermethylation in the lung of the dead cloned pig,and was significantly higher than that in the control(P＜0.01).Also,the tested CpGs sites from 4 to 9 and 12 to 17 exhibited full methylation. There was no significant differences among other tissues in the DMR2 (P＞0.05). Results showed that the abnormal DNA methylation proflies of DMR2 may occurred in the lung of cloned pig,which may be one of the factors for the death of cloned animals.

Key words: differentially methylated regions; DNA methylation; somatic cell cloning; epigenetic reprogramming

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