Abstract: Genetic variation of Luxi,BLACK,Bohai cattle in Shandong and one foreign breed,Japanese black breed,was assessed by analyzing a random sample of 185 animals using 10 microsatellite loci. A total of 58 alleles were identified, averaged about 5.8 ranging from 4 (BM1818) to 8 (ETH225 and TGLA53). All the microsatellites were highly polymorphic,and various genetic variability measures,including observed heterozygosity (0.2270 to 0.5459), expected heterozygosity (0.6082 to 0.7230) and polymorphism information content (0.5482 to 0.6791)， and effective numbers of alleles was 2.5417 to 3.5849. Considerable genetic differentiation was observed and 34.49% of the total genetic variation originated from the differences among breeds. Then we constructed UPGMA tree by genetic distances (D_A). Luxi cattle clustered first with Bohai breed, then BLACK cattle and Japanese black clustered.The sequences of PCR amplification fragment of 10 clones of 3 microsatellite loci in BLACK cattle were accepted by GenBank, the GenBank accession numbers were GQ368896,GQ368897,GQ368898,GQ368899,GQ368900,GQ368901,GQ368902,GQ368903,GQ368904,GQ368905,respectively. All of 10 microsatellite loci were effective markers for analysis of genetic relationship among 4 beef cattle breeds.

Key words: BLACK beef cattle; genetic diversity; microsatellite; clone