Abstract: In order to increase the quality of transgene vectors and the transgenic efficiency, the construction and the development course of the lentiviral vector were reviewed. In order to increase the biosafety, lengthen the loaded fragment of target DNA, and extend the host cell of the lentiviral vector, all accessory genes of lentivirus were deleted, certain exogenous regulatory sequences were introduced, and the envelope glycoprotein was replaced also, which gives the lentiviral vector a broad range of target cells and a higher biosafety, transfer efficiency and expression efficiency. What’s more, the developed transfection method laid the foundation for integration and optimization of transgenic technology. The integration and optimize between lentiviral vector and other transgene technologies will help to create a simple, efficient and economical transgene technology.

Key words: transgene; lentiviral vector; strategy of vector construction