›› 2010, Vol. 37 ›› Issue (4): 33-36.

• 生理生化 • Previous Articles     Next Articles

A Comparative Study of NO and ET-1 on the Effects of Rat Myocardial Microvascular Endothelial Cell Proliferation

JIANG Xiao-lin1， ZHANG Tao2，SUO Zhan-wei1，DONG Hong2，YI Peng-fei3，GUO Yang3， MU Xiang1,2   

  1. (1.Department of Animal Science and Technology, Beijing Agricultural College, Beijing 102206, China; 2.Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing 102206, China; 3.Institute of Animal Husbandry and Veterinary Medicine, Jilin University, Changchun 130062, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-04-20 Published:2010-04-20
  • Contact: MU Xiang

Abstract: To compare the effects of ET-1 and S-Nitroso-N-acetyl penicillamine (SNAP), the donor of NO, on the proliferation of rat myocardial microvascular endothelial cells (RMMVECs). RMMVECs were incubated with different concentrations of SNAP and ET-1 for 12 h. The cell morphology was observed and cell proliferation ability was determined by MTT. Compared with the control group, the inhibition ratio of cell proliferation of various concentrations of SNAP were 10.74% to 18.80%(P<0.05), while for the various concentrations of ET-1, they could promote the ratio of cell proliferation significantly（P<0.05 or P<0.01）, the promotion ratio were 20% to 42%. NO could inhibit the proliferation of RMMVECs in vitro, while ET-1 could promote it, which implied that the balance of NO and ET-1 played an important role in the development and treatment of diseases in vivo.

Key words: NO; ET-1; microvascular endothelial cells; proliferation

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