犬细小病毒北京分离株VP2基因的表达与抗原性分析

›› 2010, Vol. 37 ›› Issue (4): 185-186.

• 疾病防治 • Previous Articles Next Articles

Prokaryotic Expression and Analysing Antigenicity of VP2 Gene Protein of CPV

ZENG Ni, LI Gang，ZHU Hong-fei，HOU Shao-hua，SHI Li-jun

(Institute of Animal Science and Veterinary Medicine, Chinese Academy of Agricultural Sciences， Beijing 100193, China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-04-20 Published:2010-04-20
Contact: SHI Li-jun

Abstract

Abstract: One specific pairs of primers, which were designed by canine parvovirus(CPV) VP2 gene sequence, were used to clone the VP2 gene isolated from Beijing through PCR. VP2 gene was subcloned from pEASY-T-VP2 into prokaryotic expression vector pET-32a. Sequence analysis confirmed that the VP2 gene was inserted correctly. SDS-PAGE and Western-blotting analysis revealed that the VP2 protein was expressed at high level in Escherichia coli. Through ELISA assay, the expressed VP2 protein has fine immunogenicity.

Key words: canine parvovirus; expression; immunogenicity

CLC Number:

S852.65⁺9.2

ZENG Ni;LI Gang;ZHU Hong-fei;HOU Shao-hua;SHI Li-jun. Prokaryotic Expression and Analysing Antigenicity of VP2 Gene Protein of CPV[J]. , 2010, 37(4): 185-186.

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Prokaryotic Expression and Analysing Antigenicity of VP2 Gene Protein of CPV

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