Abstract: The Dezhou donkey heart fatty acid-binding protein (H-FABP) gene was cloned and compared the nucleotide sequence of coding region and amino-acid sequences with the homologous H-FABP genes in other eleven species retrieved from GenBank . The results showed that the Dezhou donkey H-FABP gene had four exons and three introns. The size of exon 1，exon 2，exon 3 and exon 4 were 73，173，102 and 57 bp respectively; while the size of intron 1，intron 2 and intron 3 were 3500，1981 and 1425 bp respectively. The size of CDS sequence of the Dezhou donkey H-FABP gene was 405 bp,which encoding 134 amino acids. The nucleotide sequences among different species were quite conservative. The homologies of the coding region of H-FABP genes between the Dezhou donkey and horse，Sus scrofa，human，cattle，goat，yak，rat，mouse，Gallus gallus，Anas platyrhynchos and zebra-fish were 96.79%，91.36%，89.14%，88.89%，88.89%，88.64%，84.07%，83.46%，75.80%，74.81% and 67.90% respectively. The phylogenetic trees were constructed by ME，NJ and UPGMA methods using the coding region DNA sequences of the H-FABP genes in all species. The results indicated that the molecular phylogenetic trees were basically coincident. This result of phylogenetic clustering was identical to the zoological classification system，indicating that the H-FABP gene was also fit to construct molecular phylogenetic tree among different species.

Key words: Dezhou donkey; heart fatty acid-binding protein gene; cloning; sequence analysis; molecular phylogenetic evaluation