猫杯状病毒全基因组的克隆及序列分析

›› 2010, Vol. 37 ›› Issue (3): 109-111.

• 生物技术 • Previous Articles Next Articles

Cloning and Sequence Analysis of Feline Calicivirus Complete Genome

ZHENG Cui-ling^1,2,3, XIANG Hua^1,3, XUAN Hua^1,3, WANG Yan-shu^1,3, YAN Ti-kun^1,3

(1.Institute of Veterinary Medicine GAAS, Guangzhou 510640, China; 2.Tangshan Vocational Technical College of Hebei, Tangshan 063004, China; 3.College of Veterinary Medicine Jilin University, Changchun 130062, China）

Received:1900-01-01 Revised:1900-01-01 Online:2010-03-20 Published:2010-03-20
Contact: XIANG Hua

Abstract

Abstract: Complete sequence of feline calicivirus (FCV) CH-GD strain was amplified by RT-PCR and recombinant PCR. DNAStar software was used to analyse nucleotide sequences and amino acid sequences. The homology of CH-GD strain which was isolated first in Guangdong province of China with reference strains was between 75.4% and 77.1%. It was lower than that was between reference strains（78.9% and 99.9%）. There were two branches between 14 strains. CH-GD strain was in one branch and there was no evident difference in district. Analysing six regions of FCV capsid protein, the characteristic of A-F region was equal to that had been reported. Antigenicity and hydrophilicity were different from reference strains in three regions where three consecutive amino acids were variation.

Key words: feline calicivirus; complete genome; clone; sequence analysis

CLC Number:

ZHENG Cui-ling;;XIANG Hua;XUAN Hua;WANG Yan-shu;YAN Ti-kun;. Cloning and Sequence Analysis of Feline Calicivirus Complete Genome[J]. , 2010, 37(3): 109-111.

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Cloning and Sequence Analysis of Feline Calicivirus Complete Genome

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