Abstract:

Abstract: Experiment was designed to culture pulmonary artery smooth muscle cells of broiler with the enzymaic digestion method.Pulmonary artery media layer from 1 to 7 day old broilers was cut into less than 1 mm×1 mm pieces， and was digested for pulmonary artery smooth muscle cells by 0.1％ collagenase type Ⅱ. The obtained cells were then cultured and subcultured. Identifications of the cells were performed with the morphological feature and immunohistochemical stain using monoclonal antibody against smooth muscle αaction.The results showed that live cells and HE stained cells were typical spindleshaped and overlapping layer form of vascular smooth muscle cells under phase contract and light microscope. αactin positive stained cells were more than 97％.Therefore，the results showed that 0.1％ collagenase type Ⅱcould digest and isolate broiler PASMC. This method for culture of PASMC has advantages of easy performance, more living cells and short period for passaging．This method can be used for study in cadiovascular disease such as pulmonary hypentension and pulmonary vascular structural remodelling．

Key words: Key words: pulmonary artery smooth muscle cell; cell culture; collagenase type Ⅱ