不同培养液对体外培养的小鼠胸腺上皮细胞增殖的影响

China Animal Husbandry & Veterinary Medicine ›› 2010, Vol. 37 ›› Issue (10): 229-231.

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The Effects of Different Medium on Proliferation Activities of Cultured Mouse Thymic Epithelial Cells

LI Ying, MA Yong-jiang, LIU Jian-hong, ZHANG Yuan, LI Yu-gu

(College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-10-20 Published:2010-10-20
Contact: LI Yu-gu

Abstract

Abstract: To investigate the effects of DMEM, DMEM/F12, RPMI 1640 on the proliferation activities of cultured mouse thymic epithelial cells (mTECs), the proliferation of the mTECs was measured by MTT method, and the cell cycle of the mTECs was examined by flow cytometer. The results showed that the proliferation activity and proliferation index of the mTECs were significantly higher in DMEM than that in DMEM/F12 or RPMI 1640 medium. It indicated that DMEM is the best medium for mTEC culture.

Key words: mouse thymic epithelial cells (mTECs); medium; proliferation; cell cycle

CLC Number:

S852.16⁺7

LI Ying;MA Yong-jiang;LIU Jian-hong;ZHANG Yuan;LI Yu-gu. The Effects of Different Medium on Proliferation Activities of Cultured Mouse Thymic Epithelial Cells[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(10): 229-231.

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The Effects of Different Medium on Proliferation Activities of Cultured Mouse Thymic Epithelial Cells

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