小鹅瘟病毒H株VP3基因序列分析

Abstract

Abstract: VP3 gene of goose parvovirus was amplified from the DNA by polymerase chain reaction(PCR), the VP3 gene was cloned into pMD18-T vector. The recombinant plasmid was indentified with restriction endonuclease analysis and PCR, and then sequenced. The result of sequence analysis showed that VP3 gene had 1605 bp and included a complete open reading frame which encoding a protein of 534 amino acids, and the analysis of molecular biology showed that the virus was virulent strain.

Key words: GPV; VP3 gene; sequence analysis

CLC Number:

WANG Bin;SUN De-jun;CHAI Hua;WANG Dan-na;PAN Xing-guang. Analyzing of the VP3 Gene Sequence of Goose Parvovirus H Strain[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(10): 155-158.

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Analyzing of the VP3 Gene Sequence of Goose Parvovirus H Strain

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