小鹅瘟病毒H株VP3基因序列分析

›› 2010, Vol. 37 ›› Issue (10): 155-158.

• 遗传繁育 • Previous Articles Next Articles

Analyzing of the VP3 Gene Sequence of Goose Parvovirus H Strain

WANG Bin, SUN De-jun, CHAI Hua, WANG Dan-na, PAN Xing-guang

(Harbin Phermaceutical Group Bio-vaccine Co.Ltd., R and D Center, Harbin 150069, China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-10-20 Published:2010-10-20

Abstract

Abstract: VP3 gene of goose parvovirus was amplified from the DNA by polymerase chain reaction(PCR), the VP3 gene was cloned into pMD18-T vector. The recombinant plasmid was indentified with restriction endonuclease analysis and PCR, and then sequenced. The result of sequence analysis showed that VP3 gene had 1605 bp and included a complete open reading frame which encoding a protein of 534 amino acids, and the analysis of molecular biology showed that the virus was virulent strain.

Key words: GPV; VP3 gene; sequence analysis

CLC Number:

Q78

WANG Bin;SUN De-jun;CHAI Hua;WANG Dan-na;PAN Xing-guang. Analyzing of the VP3 Gene Sequence of Goose Parvovirus H Strain[J]. , 2010, 37(10): 155-158.

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Analyzing of the VP3 Gene Sequence of Goose Parvovirus H Strain

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