猪乙型脑炎病毒E基因的克隆与序列分析

›› 2009, Vol. 36 ›› Issue (9): 71-73.

• 生物技术 • Previous Articles Next Articles

Cloning and Sequence Analysis of E Gene of Japanese Encephalitis Virus

CONG Xiao-yan¹, WU Jia-qiang¹, WANG Huai-zhong¹, XU Wen²,ZHANG Xiu-mei¹, WANG Jin-bao¹, CHEN Pu-yan³

（1.Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan 250100, China; 2.Qingdao Agricultural University, Qingdao 266109, China; 3.College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China）

Received:1900-01-01 Revised:1900-01-01 Online:2009-09-20 Published:2009-09-20

Abstract

Abstract:

According to the published E genes sequence of JEV in GenBank, two pairs of PCR primers were designed. E genes were amplified from JEV-SD001 strain. Then the PCR products were cloned into pMD-18 Easy Vector, and the recombinant plasmids were obtained. The sequence of the cloned PCR products were analyzed with other JEV isolates in GenBank. The results indicated that the homology of their nucleotide sequence was over 97.2%, and it shared over 96.8% of amino acid homology with other JEV isolates. JEV-SD001 belonged to Japanese encephalitis virus genotype Ⅲ, E protein of isolates showed some differences as compared with vaccine strains.

Key words: Japanese encephalitis virus; E gene; sequence analysis

CLC Number:

CONG Xiao-yan;WU Jia-qiang;WANG Huai-zhong;XU Wen;ZHANG Xiu-mei;WANG Jin-bao;CHEN Pu-yan. Cloning and Sequence Analysis of E Gene of Japanese Encephalitis Virus [J]. , 2009, 36(9): 71-73.

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Cloning and Sequence Analysis of E Gene of Japanese Encephalitis Virus

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