›› 2009, Vol. 36 ›› Issue (9): 74-78.

• 生物技术 • Previous Articles     Next Articles

Determination of Tetracyclines in Fowl Feeds and Premix by High Performance Capillary Electrophoresis

RAO Qin-xiong1, REN Lin-lin2, TONG Jing2, GUO Ping3, LI Hai-yan3, DING Shuang-yang2   

  1. (1.Institute for Agr-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201106， China； 2.National Reference Laboratories for Veterinary Drug Residue, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193， China； 3.Jiangxi Entry-Exit and Inspection and Quarantine, Nanchang 330002， China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-09-20 Published:2009-09-20
  • Contact: DING Shuang-yang

Abstract: A high performance capillaty electrophoresis (HPCE) method has been established for the separation of tetracycline1, oxytetracycline and doxycycline in fowl feeds and premix. Separation was carried out in a 64.5 cm×75 μm I.D. fused-silica uncoated capillary in 40 mmol/L Na2HPO4-20 mmol/L Citric acid buffer (pH 2.6) with 30 kV. The linear of the melamine was 1 mg/kg to 80 mg/kg, and correlation index was higher than 0.994. Limit of quantitations of feeds and premix were 2 mg/kg and 4 mg/kg, respectively. The average recoveries were higher than 80% in the spiked range from the concentration of 2 mg/kg to 80 mg/kg, and RSDs were lower than 10%. 

Key words: tetracyclinel; oxytetracycline; doxycycline; HPCE; feeds

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