Abstract: DS-ELISA was used to detect IgG antibody against HEV from 673 portions of sheep blood serum and 675 portions of cattle blood serum in 14 areas of Xinjiang, comparing the differences of antibody positive in different years of cattle and sheep and in the north and the south of Xinjiang respectively. Results showed that positive rate of HEV antibody in all tested sheep was 7.73% (52/673); the positive rate of HEV antibody in sheep was 7.61% (15/197) and 7.77% (37/476) in the south and the north of Xinjiang respectively,the difference was not significant. The difference of HEV antibody in the sheep that under 1 year and from 1 to 3 year and over 3 years was not significance(P>0.05); positive rate of HEV antibody in all tested cattle was 5.78% (39/675); the positive rate of HEV antibody in cattle was
4.19%（7/167) and 6.30%（32/508） in the south and the north of Xinjiang respectively,the difference was extremely significant. The difference of HEV antibody in the cattle that under 2 years and from 2 to 4 years and over 4 years was extremely significance(P<0.05); study indicated the infection of HEV nomally exist in sheep and cattle of Xinjiang.

Key words: HEV; DS-ELISA; sheep; cattle