Abstract: To search the better extender, cryoprotectant and thaw temperature for cryopreservate domestic cat epididymal spermatozoa, the domestic cat epididymal spermatozoa was collected and assessed before and after rapid cryopreservation by two different extenders and three different cryoprotectants respectively. Then, they were thawed in warm water bath either at 37 ℃ or 30 ℃. The results showed that extender II was much more effective than another one. As a cryoprotectant, the glycol was also much better than the glycerol and DMSO（P<0.05）. The post-thawing motility and abnormal sperm rate of the frozen/thawed spermatozoa protected by glycol reached 52.7%±4.9% and 37.3%±4.2% respectively. Its intact acrosome rate was 52.4%±4.1%, but there was no significant difference between the group with glycerol and that with DMSO. It indicated that the glycol and extender II was better for cryopreservate domestic cat epididymal spermatozoa, thawing effect at 37 ℃ was much better than that at 30 ℃.

Key words: domestic cat; epididymal spermatozoa; cryopreservation