Abstract: Porcine reproductive and respiratory syndrome is an immunosuppressive contagious disease of swine, which is one of major diseases seriously harmed to swine industry. Making an accurate diagnosis by various detection techniques is the prerequisite of preventing and controlling this disease. The detection techniques include virus detection, antibody detection and typing diagnosis, involving viral isolation, immunohistochemistry, RT-PCR, indirect immunofluorescence, immunoperoxidase monolayer assay, enzyme linked immunosorbent assay, gene array etc. Each of above techniques has its own advantage and limitation, the principle, method and character of each technique were reviewed and compared, the application and development were also prospected.

Key words: porcine reproductive and respiratory syndrome virus; diagnosis; detection technique