瘤胃微生物定量方法的研究进展

›› 2009, Vol. 36 ›› Issue (4): 19-24.

• 动物营养与饲料科学 • Previous Articles Next Articles

Research Progress on Quantitative Method for Rumen Microorganism

GUO Tong-jun1,2,WANG Jia-qi1,WANG Jian-ping1,HUO Xiao-kai1,2,BU Deng-pan1,WEI Hong-yang1,ZHOU Ling-yun1,LIU Kai-lang1

(1.State Key Laboratory of Animal Nutrition ，Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193，China;2. Xinjing Agricultural University, Urumqi 830052,China)

Received:1900-01-01 Revised:2008-11-26 Online:2009-04-20 Published:2009-04-20
Contact: WANG Jia-qi

Abstract

Abstract: There are only 10%～20% microbes being learned form rumen by now. It is the necessary for promoting extensive and intensive research on rumen ecosystem to develop novel research method. This review discuss about the researched progress and their advantage or disadvantage of the quantitative method of traditional (roll-tube technique and most probable number) and modern molecular biology (nucleic acid hybridization, FISH, DGGE, quantification PCR and Flow cytometry).

Key words: rumen microorganism; roll-tube technique; most probable number; Real-Time PCR; flow cytometry

CLC Number:

Q93.3

GUO Tong-jun;;WANG Jia-qi;WANG Jian-ping;HUO Xiao-kai;;BU Deng-pan;WEI Hong-yang;ZHOU Ling-yun;LIU Kai-lang. Research Progress on Quantitative Method for Rumen Microorganism[J]. , 2009, 36(4): 19-24.

[1]	YANG Min, GAO Yu, XU Zihao, ZHANG Jiulong, DELINUER·Baishanbieke, REN Wanping. Effects of Feeding Steam-flaked Corn on Weight Gain,Rumen Fermentation and Microbial Diversity of Xinjiang Brown cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2961-2970.
[2]	MA Baoyi, SHENG Chenyan, LIU Hongying, MA Qingxia, WANG Tingting, LI Jianming, SHI Kun, DU Rui, LENG Xue. Establishment of a Duplex TaqMan Real-time PCR Method for Detection of Bovine Viral Diarrhea Virus Types 1 and 2 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2326-2335.
[3]	LI Changyang, GAO Bingnan, REN Yulong, ZHANG Hao, SUN Yu, WEI Xiao, WANG Qiuju. Isolation,Identification and Biochemical Characteristics Analysis of Bovine Rumen Functional Bacteria [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1757-1764.
[4]	LI Xi, MAO Yangyi, LUO Huidi, ZHAO Peng, LI Jun, ZHANG Li, LI Chunyan, WANG Honghao. Effects of Dietary Crude Fiber Level on Rumen Microbial Composition and Diversity of Fattening Lambs [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1251-1263.
[5]	LI Jianmei, JI Zhengjian, LIU Mei, SHEN Xinyue, CHENG Xu, YU Yan, DAI Yabin. Expression Changes of Immune-related Genes in Tibetan Chickens and Recessive White Chickens Following Eimeria tenella Infection [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1440-1448.
[6]	GUO Xu, ZHANG Ke, CHEN Yulin, YANG Yuxin. The Colonization Process and Regulation Measures of Rumen Microorganisms in Young Ruminants [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 882-892.
[7]	JIANG Hui, LIU Pan, LI Yuanyuan, LIU Kuiguo, HUANG Xixia, LIU Jiangwei, XU Lei, DU Jianwen, SANG Zhagen, YANG Xuemei, ZHAO Fanfan, WANG Dan. Expression and Bioinformatics Analysis of Mastitis Resistance Related Genes in Xinjiang Brown Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 90-97.
[8]	XU Min, XU Houqiang, CHEN Wei, YANG Yang. Study on Expression of the Relate Genes Involved in Intramuscular Preadipocyte Differentiation in Congjiang Xiang Pig [J]. , 2018, 45(9): 2492-2499.
[9]	ZHAO Xueli, LIU Yi, BAN Fuguo, YAN Ruoqian, WANG Huajun, WANG Shujuan, MA Zhenyuan, WANG Dongfang. Establishment and Application of A Double TaqMan MGB Real-time PCR Assay for Detection of Canine Distemper Virus and Canine Parvovirus [J]. , 2018, 45(8): 2086-2094.
[10]	LAN Desong, GU Guibo, HOU Zhengzhong. Development of a TaqMan Real-time PCR for Differentiation of Wild-type Strain from gE-deleted Vaccine Strain of Pseudorabies Virus [J]. , 2018, 45(7): 1804-1812.
[11]	GAO Xintao, JIA Hong, HOU Shaohua, GUO Xiaoyu, YUAN Weifeng, JIANG Yitong, ZHU Hongfei, XIN Ting. Research on the Potential of IL-17 as a Biomarker of Bovine Tuberculosis and Establishment of Its Real-time PCR Assay [J]. , 2018, 45(6): 1447-1453.
[12]	MAO Ning, WANG Jiafu, ZHANG Fuping, TANG Liangting, RUAN Yiqi, YI Fanli, RAN Xueqin. Study on Differential Expression of StAR and CYP11A1 Genes in Xiang Pig Ovary [J]. , 2018, 45(5): 1137-1144.
[13]	WAN Chunhe, LIU Rongchang, CHENG Longfei, SHI Shaohua, FU Guanghua, CHEN Hongmei, FU Qiuling, HUANG Yu. Establishment of a TaqMan-based Real-time PCR Method of Duck Adenovirus A [J]. , 2018, 45(5): 1341-1348.
[14]	ZHANG Mengyao, YANG Feng, LIU Kaidong, CHENG Ming, WANG Guoyi, LIU Jifeng, LIU Nan, HE Jianning. Study on Construction of DKK1 Gene Recombinant Plasmid in Aohan Fine Wool Sheep and Its Expression in Fibroblasts [J]. , 2018, 45(1): 131-139.
[15]	BU Xiao-yan, LI Hui, SHI Xiao-li, SHI Xin-cai, ZHAO Zhong-hai, CHEN Lin. Study on Different Expression of HO-1 Gene of 70-weeks-old Changshun Blue-shelled Chicken in Different Tissues [J]. , 2017, 44(6): 1762-1768.

Research Progress on Quantitative Method for Rumen Microorganism

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