tPA信号肽和猪繁殖与呼吸综合征病毒ORF5基因原核表达载体的构建

›› 2009, Vol. 36 ›› Issue (3): 58-62.

• 生物技术 • Previous Articles Next Articles

Construction of Prokaryotic Expression Vector of PRRSV ORF5 Gene and tPA Signal Peptide

QIU Huang, XIA Ping-an, LU Gao-feng, WANG Zhong-ming, LIU Ming-li, 
LI Wei-juan, DANG Zhan-guo

(The College of Animal Husbandry and Veterinary, Henan Agricultural University, Zhengzhou 450002, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-03-20 Published:2009-03-20
Contact: XIA Ping-an

Abstract

Abstract: According to the published gene sequence of tissue plasminogen activator (tPA) from GenBank (accession number E00896), with on-line signal peptide analysis tools to predict the tPA signal peptide including 63 bp, one pair of specific primer of tPA signal peptide was designed and synthesized and the tPA signal peptide was amplified by overlapping PCR. Finally, the tPA signal sequence was introduced to replace the original signal peptide of the ORF5 gene by another pair of primer. The studies lay foundations for further study on the secretion expression and the vaccine or diagnosis kit for PRRSV.

Key words: PRRSV; ORF5 gene; tPA signal peptide; prokaryotic expression vector

CLC Number:

QIU Huang;XIA Ping-an;LU Gao-feng;WANG Zhong-ming;LIU Ming-li;LI Wei-juan;DANG Zhan-guo . Construction of Prokaryotic Expression Vector of PRRSV ORF5 Gene and tPA Signal Peptide[J]. , 2009, 36(3): 58-62.

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Construction of Prokaryotic Expression Vector of PRRSV ORF5 Gene and tPA Signal Peptide

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