骨骼肌电穿孔转基因技术研究进展

›› 2009, Vol. 36 ›› Issue (12): 187-191.

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Advance on Electroporation for Gene Transfer to Skeletal Muscles

PI Wen-hui, SONG Zhi-qiang, JI Yong, LIU Shou-ren

(Animal Husbandry & Veterinary Institute of Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-12-20 Published:2009-12-20
Contact: LIU Shou-ren

Abstract

Abstract: At the present, electroporation of skeletal muscle has been used extensively to transfer a number of functional genes in multiple large animal models. Electroporation greatly increases transfection efficiency of nonviral-vector gene in skeletal muscle. The possible mechanism was discussed on electro-gene-transfer across the cell membrane and the critical factors were analyzed which had a full impact on skeletal electro-gene-transfer efficacy include species, cell size, field strength, pulse parameters, electrodes and electroporater. 

Key words: in vivo electroporation; skeletal muscle; plasmid

CLC Number:

Q789

PI Wen-hui;SONG Zhi-qiang;JI Yong;LIU Shou-ren. Advance on Electroporation for Gene Transfer to Skeletal Muscles[J]. , 2009, 36(12): 187-191.

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Advance on Electroporation for Gene Transfer to Skeletal Muscles

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