Abstract: Research on the molecular level from sheep breeds of genetic diversity between resources for the comprehensive evaluation of germplasm resources in Xinjiang local sheep to provide a basis. Screened with eight pairs of E+3/M+3 primer place on the six breeds of sheep genomic DNA for AFLP amplification. According to the AFLP analysis, statistics for each primer combination in each species was detected in the polymorphic bands and specific bands were calculated by six sheep breeds genetic similarity coefficients and genetic distance and UPGMA clustering to build a relationship Fig. The results showed that 8 pairs of primer combinations get total of 334 clearly identifiable bands, it means primer combinations generated 41.75 polymorphic markers, and obtains 103 polymorphic bands, percentage of polymorphic loci was 30.838%, while all varieties also detect specific bands. The relationship of sheep Kazak recently with Altai (0.907), Kerkezi sheep was the longest Kazak (0.437), The six sheep of the local breeds are divided into 3 categories.So the six sheep of local breeds have abundant genetic diversity, genetic similarity coefficients and clustering results to the geographical distribution of sheep breeds, coincide with the actual situation, the surface of AFLP markers for genetic diversity analysis of sheep place is feasible.

Key words: local sheep breeds; AFLP; genetic diversity; relationship