绒山羊细菌人工染色体BAC文库构建条件的优化

›› 2009, Vol. 36 ›› Issue (10): 55-58.

• 生物技术 • Previous Articles Next Articles

Optimizing the Constructing Condition of Bacterial Artificial Chromosome Library in the Cashmere Goat

LIU Zhi-hong¹, LI Ning², REN Li-ming², HU Xiao-xiang², GUO Ying², 
CUI Wen-tao³, YIN Jun¹, ZHANG Wen-guang¹, LI Jin-quan^1

(1.College of Animal Science and Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China; 2.State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China; 3.Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-10-20 Published:2009-10-20

Abstract

Abstract: In recent years, bacterial artificial chromosome (BAC) library has become one of the most popular used genome library, and it is used generally in the research of eukaryotic organism that has longer genome by its advancement including larger capacity, stable inherited characteristic, few chimera, easily reclaiming insertion element, convenient operation and so on. This article researched the constructing condition of BAC library, cutting the goat’s chromosome by HindⅢ enzyme, separating the big DNA fragment by pulsed field gel electrophoresis, reclaiming DNA by electrodialysis, linking with BAC bearer taken off phosphorous, transforming competent cell DH10B by elutriations, and finally evaluating the length of fragment by cutting the DNA with NotⅠenzyme. The consequence indicated that the method that this research chose can get not only the longer insertion element, but also the higher converting efficiency, and it is the better method to construct the bigger genome BAC library.

Key words: bacterial artificial chromosome; library; constructing condition

CLC Number:

S827.2

LIU Zhi-hong;LI Ning;REN Li-ming;HU Xiao-xiang;GUO Ying;CUI Wen-tao;YIN Jun;ZHANG Wen-guang;LI Jin-quan. Optimizing the Constructing Condition of Bacterial Artificial Chromosome Library in the Cashmere Goat[J]. , 2009, 36(10): 55-58.

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Optimizing the Constructing Condition of Bacterial Artificial Chromosome Library in the Cashmere Goat

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