口蹄疫病毒VP1基因的原核可溶性表达

›› 2009, Vol. 36 ›› Issue (10): 50-54.

• 生物技术 • Previous Articles Next Articles

The Exploration of Prokaryotic Soluble Expression of Foot and Mouth Disease Virus VP1 Gene

WANG Hai-feng^1,2, YI Zhong¹，WEI Yu-rong¹, WEI Jie^1,2, HUERMAXI¹,FU Zi-hua¹

（1.Veterinary Research Institute, Xinjiang Academy of Animal Sciences, Urumqi 830000, China；2.Animal Medical College， Xinjiang Agriculture University, Urumqi 830052, China）

Received:1900-01-01 Revised:1900-01-01 Online:2009-10-20 Published:2009-10-20
Contact: FU Zi-hua

Abstract

Abstract: Owing to the recombinant prokaryotic expression vector to express the target protein as inclusion body, VP1 protein was induced by different concentrations of IPTG，different temperatures and different time，cell lysates were analyzed by SDS-PAGE, to observe whether emerge soluble protein. The VP1 gene was amplified by PCR and cloned into pGEM-T vector, then pET-28a(+) and pGEM-T vector were dual-Enzyme digested respectively, a recombinant of pET-28a-VP1 vector was achieved and transformed into BL21. The results showed that protein expression yield was impacted by IPTG concentration and temperature and time, but the protein mainly was formed of inclusion bodies. The results show that inclusion body may be related to the amino acid composition.

Key words: FMDV; VP1 gene; prokaryotic; soluble expression

CLC Number:

S852.65⁺9.6

WANG Hai-feng;YI Zhong;WEI Yu-rong;WEI Jie;HUERMAXI;FU Zi-hua. The Exploration of Prokaryotic Soluble Expression of Foot and Mouth Disease Virus VP1 Gene[J]. , 2009, 36(10): 50-54.

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The Exploration of Prokaryotic Soluble Expression of Foot and Mouth Disease Virus VP1 Gene

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