Abstract: Norovirus is an important zoonotic pathogen threatening human health. To serve as positive control for the molecular biological examination of norovirus infection and facilitate deducing the origin of this isolate, the total RNA of a feces sample was extracted and used as template for reverse transcription. The first strand cDNA was amplified by 3’RACE (rapid amplification of cDNA 3’ends) using gene specific primer JV12 as forward inner primer and 3’RACE backward inner primer. The amplicon was 3282 bp in length judged from agarose electrophoresis. The amplicon was ligated to the Promega Teasy vector and sequence determined. The determined nucleotide sequence was then analyzed by blast online. Sequence analysis indicated that 〖JP2〗the isolated virus strain belonged to Norovirus GⅡ4 cluster and has the same origin with the isolate in Japan. 3’RACE 〖JP〗amplification established a basis for experimental detection and further research of Norovirus.

Key words: norovirus; 3’RACE; sequence analysis; origin