抗菌肽Cecropin P1基因真核表达载体的构建

›› 2007, Vol. 34 ›› Issue (9): 58-60.

• 生物技术 • Previous Articles Next Articles

Construction of Eukaryotic Expression Vector of Antimicrobial Peptide Gene Cecropin P1

LI Jianxia1,2, MIAO Xiangyang2, DING Zhaozhong2 , REN Huiying1,2, YU Zhongna1,2

1.Animal Science and Technology Department of Laiyang Agriculture College, Chengyang 266109，China; 2.Institute of Animal Sciences，Chinese Academy of Agriculture Sciences, State Key Laboratory of Animal Nutrition, Beijing 100094,China

Received:1900-01-01 Revised:1900-01-01 Online:2007-09-20 Published:2007-09-20

Abstract

Abstract: Three gene fragments were designed and synthesized according to the preference of E.coli.The gene of Cecropin P1 was amplified by PCR. The target gene fragment modified was cloned into vector T-easy, and then inserted into expression plasmid pcDNA3.1(+),the expression vector construction was completed. It was confirmed that the rombinant plasmid was successfully constructed by restriction endonuclease analysis and DNA sequencing, This may lay a foundation in research of antimicobial activities and antimicrobial mechanism in the gene of Cecropin P1.

Key words: Cecropin P1; construction of expression vector; antimicrobial peptide

CLC Number:

Q782

LI Jianxia;MIAO Xiangyang; DING Zhaozhong;REN Huiying;YU Zhongna;. Construction of Eukaryotic Expression Vector of Antimicrobial Peptide Gene Cecropin P1[J]. , 2007, 34(9): 58-60.

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Construction of Eukaryotic Expression Vector of Antimicrobial Peptide Gene Cecropin P1

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