基于天蚕素A和胡蜂毒素的杂合肽设计及活性鉴定

doi:10.16431/j.cnki.1671-7236.2020.09.036

Abstract

Abstract: In order to further improve mastoparan's antibacterial and antitumor activities and reduce its cytotoxicity,the molecular modification was carried out by molecular hybridization,and the biological activity of the designed peptide was identified.A novel hybrid peptide KL-21 was obtained by using the N-terminal 1-8 sequences fragment of cecropin A and the conserved sequence of mastoparan.The physicochemical parameters and structures of KL-21 were analyzed by bioinformatics method.The antibacterial activity of KL-21 was studied with MP-L as reference.In addition,the hemolysis rate of the two peptides and the activity of anti-tumor cell A549 were measured.The results showed that KL-21 could quickly kill bacteria in a shorter time.The minimum inhibitory concentration (MIC) of KL-21 to Gram-positive bacteria (S.aureus) and Gram-negative bacteria (E.coli) was 4-8 μg/mL,and the minimum bactericidal concentration (MBC) was 8-16 μg/mL.The MIC and MBC of KL-21 to fungus (C.albicans) were 32 and 64 μg/mL,respectively.The hemolytic activity of KL-21 was only 20% at 128 μg/mL,which was lower than that of MP-L.KL-21 could inhibit lung cancer cell A549 with inhibition rate in vitro about 86% at 16 μg/mL.These results indicated that KL-21 had higher antibacterial and antitumor activity and lower cytotoxicity than MP-L,which could be used as a leading peptide for further research and development.

Key words: cecropin; mastoparan; hybrid peptide; antimicrobial peptide; antitumor cell

CLC Number:

S859.79⁺7

ZHAI Pei, HAN Jinhui, PAN Xiaoyu. Design and Activity Identification of Hybrid Peptide Based on Cecropin A and Mastoparan[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 3006-3013.

References

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Design and Activity Identification of Hybrid Peptide Based on Cecropin A and Mastoparan

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