水貂FSH-β基因序列的克隆与测序

Abstract

Abstract: The author separates and extracts the overall DNA from leg muscles of mink, then gets the DNA sequence of mink FSH-β gene after PCR amplification. The product of PCR amplification is then conjunction onto pMD-18T carrier after gelatum reclaim, then transformed into competence cell JM109. bolting masculine clone colony on LB(Amp+) medium which contains X-gal and IPTG. Then after plasmid extraction， restriction enzyme cut and PCR accredit, the target segment is sequencing. As a result of the test, the amount of basic group of DNA segment after PCR amplification is 500bp, by contrast with Blast, the author analyzes the home ology of follicle-stimulating hormone ribonucleotide sequence between mink and human being, ox, pig, sheep, mouse.

Key words: mink; FSH-β gene; clone; sequence analysis

CLC Number:

Q785

LI Yumei;BAI Xiujuan. Cloning and Sequencing on the Gene Sequence of Mink FSH-β Hormone [J]. , 2007, 34(9): 61-63.

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Cloning and Sequencing on the Gene Sequence of Mink FSH-β Hormone

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