单精注射法生产转GFP基因猪胚胎的研究

China Animal Husbandry & Veterinary Medicine ›› 2007, Vol. 34 ›› Issue (6): 44-46.

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Production of Porcine Transgenic Embryos Expressing GFP by Intracytoplasmic Sperm Injection(ICSI)

MIAO Xiangyang1,FANG Changge1,2,SHAO Jianjun1,ZHU Ruiliang3,REN Huiying1

1.Institute of Animal Science,Chinese Academy of Agricultural Science,Beijing 100094,China; 2.The State Key Laboratory for AgroBiotechnology, China Agricultural University, Beijing 100094,China; 3.College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, China

Received:1900-01-01 Revised:1900-01-01 Online:2007-06-20 Published:2007-06-20

Abstract

Abstract: Intracytoplasmic sperm injection(ICSI), which is a new technique of embryo engineering of domestic animals, can solve the problem of porcine polyspermy. Porcine ICSI was conducted using repeatedly frozen/thawed intact sperm or sperm head which were incubated with GFP gene for 30 minutes.The expression rate of GFP gene in ICSI oocytes activated by CaCl2 was higher than that of ICSI oocytes activated by electrical pulse. The result showed that SMGT-ICSI procedure for the production of transgenic porcine embryos was feasible.

Key words: oocytes; ICSI; embryo

CLC Number:

S814.8

MIAO Xiangyang;FANG Changge;SHAO Jianjun;ZHU Ruiliang;REN Huiying . Production of Porcine Transgenic Embryos Expressing GFP by Intracytoplasmic Sperm Injection(ICSI)[J]. China Animal Husbandry & Veterinary Medicine, 2007, 34(6): 44-46.

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Production of Porcine Transgenic Embryos Expressing GFP by Intracytoplasmic Sperm Injection(ICSI)

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