Abstract: This paper focused on isolated primordial germ cells (PGCs) from gonads at stage 19 by Ficoll densitygradient centrifugation. Then, the PGCs were froze respectively in six kinds of freezing medias under slow speed cryopreservation process and vitrification process. The viability of the frozenthawed PGCs was measured by the trypan blue exclusion method. The results showed: For the slow speed cryopreservation, the viability of the frozenthawed PGCs under freezing media V was the highest, and showed significant difference（P<0.05）when compared to that of freezing mediaⅠ.For the vitrificated cryopreservation, the viability of the frozenthawed PGCs under freezing media I was the highest,and showed very significant difference（P<0.01）when compared to that of other five freezing medias. The third passage PGCs subcultured after slow speed cryopreservation and the second passage PGCs subcultured after vitrificated cryopreservation were positive to PAS and AKP staining and still kept intact karyotypes. 

Key words: chicken; PGCs; slow speed cryopreservation; vitrificated cryopreserration; subculture