China Animal Husbandry & Veterinary Medicine ›› 2007, Vol. 34 ›› Issue (12): 52-55.

• 生物技术 • Previous Articles     Next Articles

Cloning and Sequence Analysis of Exon 2 of BMP15 Gene in Jining Grey Goats

JIAO Cailan1，CHU Mingxing2，WANG Jinyu1，FANG
Li2，YE Sucheng2   

  1. 1.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;2.Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-12-20 Published:2007-12-20

Abstract: The 857 bp fragment of exon 2 of bone morphogenetic protein 15（BMP15）gene was amplified successfully by PCR in both 10 does of high prolificacy breed (Jining Grey goat) and 10 does of low prolificacy breed (Inner Mongolia Cashmere goat), and then it was cloned and sequenced. The results showed that the differences between Jining Grey goat and Inner Mongolia Cashmere goat consisted of two nucleotide and two amino acid changes and the homology of nucleotide sequence between them was 99%. Homologies of nucleotide sequence and amino acid sequence of exon 2 of BMP15 gene between Jining Grey goat and chicken, house mouse,human, pig, cattle, sheep were 71% to 99% and 43% to 99%, respectively.

Key words: goat; prolificacy; bone morphogenetic protein-15 gene; cloning; sequence analysis

CLC Number: