Abstract: The 857 bp fragment of exon 2 of bone morphogenetic protein 15（BMP15）gene was amplified successfully by PCR in both 10 does of high prolificacy breed (Jining Grey goat) and 10 does of low prolificacy breed (Inner Mongolia Cashmere goat), and then it was cloned and sequenced. The results showed that the differences between Jining Grey goat and Inner Mongolia Cashmere goat consisted of two nucleotide and two amino acid changes and the homology of nucleotide sequence between them was 99%. Homologies of nucleotide sequence and amino acid sequence of exon 2 of BMP15 gene between Jining Grey goat and chicken, house mouse,human, pig, cattle, sheep were 71% to 99% and 43% to 99%, respectively.

Key words: goat; prolificacy; bone morphogenetic protein-15 gene; cloning; sequence analysis